Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes

• DOI: 10.1038/s41467-020-16488-y
• Publication Date: 2020-05-27T10:03:12Z
• Authors: Shuhei Yoshida; Sui Nishiyama; Lisa Lister; Shu Hashimoto; Tappei Mishina; Aurélien Courtois; Hirohisa Kyogoku; Takaya Abe; Aki Shiraishi; Meenakshi Choudhary; Yoshiharu Nakaoka; Mary Herbert; Tomoya S. Kitajima
• Keywords: 0301 basic medicine; Science; Q; Nuclear Proteins; Cell Cycle Proteins; Spindle Apparatus; Microtubules; Article; Gametogenesis; Cytoskeletal Proteins; Meiosis; Mice; 03 medical and health sciences; Chromosome Segregation; Oocytes; Animals; Female; Kinetochores; Microtubule-Associated Proteins

Abstract Acentrosomal meiosis in oocytes represents a gametogenic challenge, requiring spindle bipolarization without predefined bipolar cues. While much is known about the structures that promote acentrosomal microtubule nucleation, less mediate mammalian oocytes. Here, we show mouse oocytes, kinetochores are required for I. This process promoted by oocyte-specific, microtubule-independent enrichment of antiparallel crosslinker Prc1 at via Ndc80 complex. In contrast, II, cytoplasm contains upregulated factors including supports kinetochore-independent pathways bipolarization. The kinetochore-dependent mode I to prevent chromosome segregation errors. Human where reportedly error prone, exhibit no detectable kinetochore Prc1. study reveals an oocyte-specific function mice, and provides insights into error-prone nature human