Cross-serotype protection against group A Streptococcal infections induced by immunization with SPy_2191

• DOI: 10.1038/s41467-020-17299-x
• Publication Date: 2020-07-15T10:04:02Z
• Authors: Pooja Sanduja; Manish Gupta; Vikas Kumar Somani; Vikas Yadav; Meenakshi Dua; Emanuel Hanski; Abhinay Sharma; Rakesh Bhatnagar; Atul Kumar Johri
• Keywords: STREPTOCOCCAL INFECTIONS; Group A; Streptococcus Pyogenes

Group A Streptococcus (GAS) infection causes a range of diseases, but vaccine development is hampered by the high number serotypes. Here, using reverse vaccinology authors identify SPy_2191 as cross-protective candidate. From 18 initially identified surface proteins, only conserved, surface-exposed and inhibits both GAS adhesion invasion. immunization in mice generates bactericidal antibodies resulting opsonophagocytic killing prevalent invasive serotypes different geographical regions, including M1 M49 (India), M3.1 (Israel), (UK) (USA). Resident splenocytes show higher interferon-γ tumor necrosis factor-α secretion upon antigen re-stimulation, suggesting activation cell-mediated immunity. significantly reduces streptococcal load organs confers ~76-92% protection challenge with Further, it suppresses pharyngeal colonization mucosal model. Our findings suggest that can act universal candidate against infections.