Quantitative evaluation of protective antibody response induced by hepatitis E vaccine in humans

• DOI: 10.1038/s41467-020-17737-w
• Publication Date: 2020-08-07T10:03:50Z
• Authors: Gui-Ping Wen; Linling He; Zi-Min Tang; Si-Ling Wang; Xu Zhang; Yuan-Zhi Chen; Xiaohe Lin; Chang Liu; Jia-Xin Chen; Dong Ying; Zi-Hao Chen; Ying-Bin Wang; Wen-Xin Luo; Shou-Jie Huang; Shao-Wei Li; Jun Zhang; Zi-Zheng Zheng; Jiang Zhu; Ning-Shao Xia
• Keywords: Vaccination schedule; Antibody Repertoire; Antibody response

Abstract Efficacy evaluation through human trials is crucial for advancing a vaccine candidate to clinics. Next-generation sequencing (NGS) can be used quantify B cell repertoire response and trace antibody lineages during vaccination. Here, we demonstrate this application with case study of Hecolin®, the licensed hepatitis E virus (HEV). Four subjects are administered following standard three-dose schedule. Vaccine-induced antibodies exhibit high degree clonal diversity, recognize five conformational antigenic sites genotype 1 HEV p239 antigen, cross-react other genotypes. Unbiased performed seven time points over six months vaccination, maturation pathways characterize set vaccine-induced antibodies. In addition dynamic profiles, NGS analysis reveals differential patterns HEV-specific highlights necessity long boost. Together, our presents quantitative strategy in small-scale studies.