A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts

• DOI: 10.1038/s41467-020-18234-w
• Publication Date: 2020-09-10T11:26:39Z
• Authors: Youjun Zhang; Arun Sampathkumar; Sandra Mae-Lin Ke...; Corné Swart; Carsten Hille; Kumar Seerangan; Alexander Graf; Lee Sweetlove; Alisdair R. Fernie
• Keywords: Phosphoglycerate mutase; Phosphoglycerate kinase; Enolase; Mutase

Abstract Glycolysis is one of the primordial pathways metabolism, playing a pivotal role in energy metabolism and biosynthesis. Glycolytic enzymes are known to form transient multi-enzyme assemblies. Here we examine wider protein-protein interactions plant glycolytic reveal moonlighting for specific mediating co-localization mitochondria chloroplasts. Knockout mutation phosphoglycerate mutase or enolase resulted significantly reduced association two organelles. We provide evidence that substrate-channelling metabolon which part larger complex proteins including pyruvate kinase. These results alongside range genetic complementation experiments discussed context our current understanding chloroplast-mitochondrial within photosynthetic eukaryotes.