High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

• DOI: 10.1038/s41467-020-19747-0
• Publication Date: 2020-11-18T11:05:54Z
• Authors: Karl Zhanghao; Wenhui Liu; Meiqi Li; Zihan Wu; Xiao Wang; Xingye Chen; Chunyan Shan; Haoqian Wang; Xiaowei Chen; Qionghai Dai; Peng Xi; Dayong Jin
• Keywords: Organelle; Cell membrane

Abstract Lipid membranes are found in most intracellular organelles, and their heterogeneities play an essential role regulating the organelles’ biochemical functionalities. Here we report a Spectrum Polarization Optical Tomography (SPOT) technique to study subcellular lipidomics live cells. Simply using one dye that universally stains lipid membranes, SPOT can simultaneously resolve membrane morphology, polarity, phase from three optical-dimensions of intensity, spectrum, polarization, respectively. These high-throughput optical properties reveal ten compartments, at different developmental stages, even within same organelle. Furthermore, obtain real-time monitoring multi-organelle interactive activities cell division successfully sophisticated dynamics during plasma separation, tunneling nanotubules formation, mitochondrial cristae dissociation. This work suggests research frontiers correlating single-cell super-resolution with multiplexed imaging organelle interactome.