A human tissue map of 5-hydroxymethylcytosines exhibits tissue specificity through gene and enhancer modulation

• DOI: 10.1038/s41467-020-20001-w
• Publication Date: 2020-12-02T11:08:04Z
• Authors: Xiao-Long Cui; Ji Nie; Jeremy Ku; Urszula Dougherty; Diana C. West-Szy...; Francois Collin; Christopher K. El...; Laura Sieh; Yuhong Ning; Zifeng Deng; Carolyn W. T. Zhao; Anna Bergamaschi; Joel Pekow; Jiangbo Wei; Alana V. Beadell; Zhou Zhang; Geeta Sharma; Raman Talwar; Patrick Arensdorf; Jason Karpus; Ajay Goel; Marc Bissonnette; Wei Zhang; Samuel Levy; Chuan He
• Keywords: 5-Hydroxymethylcytosine

Abstract DNA 5-hydroxymethylcytosine (5hmC) modification is known to be associated with gene transcription and frequently used as a mark investigate dynamic methylation conversion during mammalian development in human diseases. However, the lack of genome-wide 5hmC profiles different tissue types impedes drawing generalized conclusions about how implicated activity specificity. To meet this need, we describe map by characterizing genomic distributions 19 tissues derived from ten organ systems. Subsequent sequencing results enabled identification that uniquely separates samples type. Further comparison transcriptomes histone modifications revealed preferentially enriched on tissue-specific bodies enhancers. Taken together, provide an extensive across diverse suggests potential role development; well resource facilitate future studies demethylation pathogenesis biomarkers.