The molecular dynamics of subdistal appendages in multi-ciliated cells

• DOI: 10.1038/s41467-021-20902-4
• Publication Date: 2021-01-27T11:06:38Z
• Authors: Hyunchul Ryu; Haeryung Lee; Jiyeon Lee; Hyuna Noh; Miram Shin; Vijay Kumar; Sejeong Hong; Jaebong Kim; Soochul Park
• Keywords: Motile cilium; Basal body; Appendage; Basal (medicine); Basal membrane; Ciliogenesis

Abstract The motile cilia of ependymal cells coordinate their beats to facilitate a forceful and directed flow cerebrospinal fluid (CSF). Each cilium originates from basal body with foot protruding one side. A uniform alignment these feet is crucial for the coordination ciliary beating. process by which subdistal appendages body, however, unresolved. Here, we show FGFR1 Oncogene Partner (FOP) useful marker delineating transformation circular, unpolarized appendage into polarized structure foot. Ankyrin repeat SAM domain-containing protein 1A (ANKS1A) interacts FOP assemble region I Importantly, disruption ANKS1A reduces size I. This produces an unstable foot, disrupts rotational polarity coordinated beating in young adult mice. deficiency also leads severe degeneration aged mice detachment bodies. role polarization evolutionarily conserved vertebrates. Thus, regulates build maintain appendages.