Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution

• DOI: 10.1038/s41467-021-20920-2
• Publication Date: 2021-01-27T11:06:38Z
• Authors: Yibin Liu; Zhiyuan Hu; Jingfei Cheng; Paulina Siejka-Zi...; Jinfeng Chen; Masato Inoue; Ahmed Ashour Ahmed; Chun-Xiao Song
• Keywords: 5-Methylcytosine; Bisulfite; 5-Hydroxymethylcytosine; Boranes; Cytosine; Bisulfite sequencing; Illumina dye sequencing

Abstract Although various methods have been developed for sequencing cytosine modifications, it is still challenging specific and quantitative of individual modification at base-resolution. For example, to obtain both true 5-methylcytosine (5mC) 5-hydroxymethylcytosine (5hmC) information, the two major epigenetic usually requires subtraction methods, which increases noise high depth. Recently, we TET-assisted pyridine borane (TAPS) bisulfite-free direct 5mC 5hmC. Here demonstrate that sister TAPSβ chemical-assisted (CAPS), can be effectively used subtraction-free whole-genome 5hmC, respectively. We also (PS) profiling 5-formylcytosine 5-carboxylcytosine, further oxidized derivatives This work completes set versatile reduction chemistry-based as a comprehensive toolkit all four modifications.