A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy

• DOI: 10.1038/s41467-021-21431-w
• Publication Date: 2021-02-19T16:46:37Z
• Authors: Junrong Li; Alain Wuethrich; Abu A. I. Sina; Han-Hao Cheng; Yuling Wang; Andreas Behren; Paul N. Mainwaring; Matt Trau
• Keywords: Immune checkpoint

Abstract The introduction of immune checkpoint inhibitors has demonstrated significant improvements in survival for subsets cancer patients. However, they carry and sometimes life-threatening toxicities. Prompt prediction monitoring toxicities have the potential to maximise benefits therapy. Herein, we develop a digital nanopillar SERS platform that achieves real-time single cytokine counting enables dynamic tracking patients receiving inhibitor treatment - broader applications are anticipated other disease indications. By analysing four prospective biomarkers initiate inflammatory responses, assay both highly specific sensitive detection down attomolar level. Significantly, report capability longitudinally monitor 10 melanoma during blockade treatment. Here, show elevated concentrations predict higher risk developing severe our pilot cohort