Functional regeneration and repair of tendons using biomimetic scaffolds loaded with recombinant periostin

• DOI: 10.1038/s41467-021-21545-1
• Publication Date: 2021-02-26T11:04:53Z
• Authors: Yu Wang; Shanshan Jin; Dan Luo; Danqing He; Chunyan Shi; Lisha Zhu; Bo Guan; Zixin Li; Ting Zhang; Yanheng Zhou; Cun-Yu Wang; Yan Liu
• Keywords: Periostin

Abstract Tendon injuries disrupt the balance between stability and mobility, causing compromised functions disabilities. The regeneration of mature, functional tendons remains a clinical challenge. Here, we perform transcriptional profiling tendon developmental processes to show that extracellular matrix-associated protein periostin (Postn) contributes maintenance stem/progenitor cell (TSPC) promotes regeneration. We recombinant (rPOSTN) proliferation stemness TSPCs, maintains tenogenic potentials TSPCs in vitro. also find rPOSTN protects against impairment during long-term passage For vivo formation, construct biomimetic parallel-aligned collagen scaffold facilitate TSPC tenogenesis. Using rat full-cut Achilles defect model, demonstrate scaffolds loaded with promote endogenous recruitment, repair native-like hierarchically organized fibers. Moreover, newly regenerated recovery mechanical properties locomotion functions.