Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology

• DOI: 10.1038/s41467-021-22308-8
• Publication Date: 2021-04-06T10:02:48Z
• Authors: Keman Cheng; Ruifang Zhao; Yao Li; Yingqiu Qi; Yazhou Wang; Yinlong Zhang; Hao Qin; Yuting Qin; Long Chen; Chen Li; Jie Liang; Yujing Li; Jiaqi Xu; Xuexiang Han; Gregory J. Anderson; Jian Shi; Lei Ren; Xiao Zhao; Guangjun Nie
• Keywords: Plug and play; Spark plug

An effective tumor vaccine vector that can rapidly display neoantigens is urgently needed. Outer membrane vesicles (OMVs) strongly activate the innate immune system and are qualified as immunoadjuvants. Here, we describe a versatile OMV-based platform to elicit specific anti-tumor response via specifically presenting antigens onto OMV surface. We first on OMVs surface by fusing with ClyA protein, then simplify antigen process employing Plug-and-Display comprising tag/catcher protein pairs. decorated different catchers simultaneously multiple, distinct synergistic antitumour response. In addition, bioengineered loaded abrogate lung melanoma metastasis inhibit subcutaneous colorectal cancer growth. The ability of may facilitate development these agents for personalized tumour vaccines.