Characterisation of tumour microenvironment remodelling following oncogene inhibition in preclinical studies with imaging mass cytometry

• DOI: 10.1038/s41467-021-26214-x
• Publication Date: 2021-10-09T07:03:53Z
• Authors: Febe van Maldegem; Karishma Valand; Megan Cole; Harshil Patel; Mihaela Angelova; Sareena Rana; Emma Colliver; Katey Enfield; Nourdine Bah; Gavin Kelly; Victoria Siu Kwan...; Edurne Mugarza; Christopher Moore; Philip Hobson; Dina Levi; Miriam Molina-Arcas; Charles Swanton; Julian Downward
• Keywords: Mass cytometry

Abstract Mouse models are critical in pre-clinical studies of cancer therapy, allowing dissection mechanisms through chemical and genetic manipulations that not feasible the clinical setting. In tumour microenvironment (TME), multiplexed imaging methods can provide a rich source information. However, application such technologies mouse tissues is still its infancy. Here we present workflow for studying TME using mass cytometry with panel 27 antibodies on frozen tissues. We optimise validate image segmentation strategies automate process Nextflow-based pipeline (imcyto) scalable portable, parallelised large multi-image datasets. With these interrogate remodelling induced by KRAS G12C inhibitor an immune competent orthotopic lung model, highlighting infiltration activation antigen presenting cells effector cells.