MCAs in Arabidopsis are Ca2+-permeable mechanosensitive channels inherently sensitive to membrane tension

• DOI: 10.1038/s41467-021-26363-z
• Publication Date: 2021-10-19T10:16:21Z
• Authors: Kenjiro Yoshimura; Kazuko Iida; Hidetoshi Iida
• Keywords: 0301 basic medicine; Cell Membrane Permeability; Arabidopsis Proteins; Science; Q; Cell Membrane; Arabidopsis; Membrane Proteins; Biological Transport; Mechanotransduction, Cellular; Article; 03 medical and health sciences; Calcium

Abstract Mechanosensitive (MS) ion channels respond to mechanical stress and convert it into intracellular electric ionic signals. Five MS channel families have been identified in plants, including the Mid1-Complementing Activity (MCA) channel; however, its activation mechanisms not elucidated detail. We herein demonstrate that MCA2 is a Ca 2+ -permeable directly activated by membrane tension. The N-terminal 173 residues of MCA1 were synthesized vitro, purified, reconstituted artificial liposomal membranes. Liposomes with MCA1(1-173) or MCA2(1-173) mediate influx application pressure elicits currents. This also voltage. Blockers for inhibit stretch, but Since MCA proteins are found exclusively these results suggest represent plant-specific open