C-Glycoside metabolism in the gut and in nature: Identification, characterization, structural analyses and distribution of C-C bond-cleaving enzymes

• DOI: 10.1038/s41467-021-26585-1
• Publication Date: 2021-11-02T11:03:37Z
• Authors: Takahiro Mori; Takuto Kumano; Haibing He; Satomi Watanabe; Miki Senda; Toshio Moriya; Naruhiko Adachi; Sanae Hori; Yuzu Terashita; Masato Kawasaki; Yoshiteru Hashimoto; Takayoshi Awakawa; Toshiya Senda; Ikuro Abe; Michihiko Kobayashi
• Keywords: Aglycone; Moiety; Bond cleavage; Enzyme Catalysis

Abstract C -Glycosides, in which a sugar moiety is linked via carbon-carbon (C-C) bond to non-sugar (aglycone), are found our food and medicine. The C-C cleaved by intestinal microbes the resulting aglycones exert various bioactivities. Although enzymes responsible for reactions have been identified, their catalytic mechanisms generality of nature remain be explored. Here, we present identification structural basis activation xenobiotic -glycosides heterocomplex -deglycosylation from soil bacteria. They metal-dependent exhibiting broad substrate specificity toward -glycosides. X-ray crystallographic cryo-electron microscopic analyses, as well structure-based mutagenesis, reveal details these detailed remarkable cleavage reactions. Furthermore, bioinformatic biochemical analyses suggest that widely distributed gut, soil, marine