Light-mediated discovery of surfaceome nanoscale organization and intercellular receptor interaction networks

• DOI: 10.1038/s41467-021-27280-x
• Publication Date: 2021-12-02T11:03:04Z
• Authors: Maik Müller; Fabienne Gräbnitz; Niculò Barandun; Yang Shen; Fabian Wendt; Sebastian N. Steiner; Yannik Severin; Stefan U. Vetterli; Milon Mondal; James R. Prudent; Raphael Hofmann; Marc van Oostrum; Roman C. Sarott; Alexey I. Nesvizh...; Erick M. Carreira; Jeffrey W. Bode; Berend Snijder; John A. Robinson; Martin J. Loessner; Annette Oxenius; Bernd Wollscheid
• Keywords: Cell Signaling

The molecular nanoscale organization of the surfaceome is a fundamental regulator cellular signaling in health and disease. Technologies for mapping spatial relationships cell surface receptors their extracellular synapses would unlock theranostic opportunities to target protein communities possibility engineer signaling. Here, we develop an optoproteomic technology termed LUX-MS that enables targeted elucidation acute interactions on between living cells using light-controlled singlet oxygen generators (SOG). By SOG-coupled antibodies, small molecule drugs, biologics intact viral particles, demonstrate ability decode ligand receptor across organisms discover with direct implications drug action. Furthermore, by coupling SOG antigens achieved intercellular within functional immune antigen-presenting CD8+ T providing insights into activation spatiotemporal specificity. based decoding architectures thereby provides framework rational development strategies.