Cell specific peripheral immune responses predict survival in critical COVID-19 patients

• DOI: 10.1038/s41467-022-28505-3
• Publication Date: 2022-02-15T11:03:01Z
• Authors: Junedh M. Amrute; Alexandra M. Perry; Gautam Anand; Carlos Cruchaga; Karl G. Hock; Christopher W. Fa...; Gwendalyn J. Rand...; Kory J. Lavine; Ashley L. Steed
• Keywords: Cell type

Abstract SARS-CoV-2 triggers a complex systemic immune response in circulating blood mononuclear cells. The relationship between cell activation of the peripheral compartment and survival critical COVID-19 remains to be established. Here we use single-cell RNA sequencing Cellular Indexing Transcriptomes Epitomes by sequence mapping elucidate type specific transcriptional signatures that associate with predict COVID-19. Patients who survive infection display antibody processing, early response, cycle regulation pathways most prominent within B-, T-, NK-cell subsets. We further leverage differential gene expression machine learning mortality using single transcriptomes. identify interferon signaling antigen presentation cDC2 cells, CD14 monocytes, CD16 monocytes as predictors 90% accuracy. Finally, validate our findings an independent transcriptomics dataset provide framework mechanisms promote critically ill patients. Identifying prognostic indicators among patients holds tremendous value risk stratification clinical management.