Revealing the human mucinome

• DOI: 10.1038/s41467-022-31062-4
• Publication Date: 2022-06-20T19:02:49Z
• Authors: Stacy A. Malaker; Nicholas M. Riley; D. Judy Shon; Kayvon Pedram; Venkatesh Krishnan; Oliver Dorigo; Carolyn R. Bertozzi
• Keywords: HAMP domain

Abstract Mucin domains are densely O-glycosylated modular protein found in various extracellular and transmembrane proteins. Mucin-domain glycoproteins play important roles many human diseases, such as cancer cystic fibrosis, but the scope of mucinome remains poorly defined. Recently, we characterized a bacterial O-glycoprotease, StcE, demonstrated that an inactive point mutant retains binding selectivity for mucin-domain glycoproteins. In this work, leverage StcE to selectively enrich identify from complex samples like cell lysate crude ovarian patient ascites fluid. Our enrichment strategy is further aided by algorithm assign confidence glycoprotein identifications. This mucinomics platform facilitates detection hundreds glycopeptides mucin highly overlapping populations patients. Ultimately, demonstrate our approach can reveal key molecular signatures vitro ex vivo sources.