Peptide fusion improves prime editing efficiency

• DOI: 10.1038/s41467-022-31270-y
• Publication Date: 2022-06-18T11:02:45Z
• Authors: Minja Velimirovic; Larissa C. Zanetti; Max W. Shen; James D. Fife; Lin Lin; Minsun Cha; Ersin Akinci; Danielle Barnum; Tian Yu; Richard I. Sherwood
• Keywords: Gene Editing; 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Science; Q; CRISPR-Cas Systems; Gene Fusion; Peptides; Article; Cell Line

Abstract Prime editing enables search-and-replace genome but is limited by low efficiency. We present a high-throughput approach, the Peptide Self-Editing sequencing assay (PepSEq), to measure how fusion of 12,000 85-amino acid peptides influences prime show that peptide can enhance editing, prime-enhancing combine productively, and top dual peptide-prime editor increases significantly in multiple cell lines across dozens target sites. Top function increasing translation efficiency serve as broadly useful tools improve