FANCM promotes class I interfering crossovers and suppresses class II non-interfering crossovers in wheat meiosis

• DOI: 10.1038/s41467-022-31438-6
• Publication Date: 2022-06-25T09:02:49Z
• Authors: Stuart D. Desjardins; James Simmonds; Inna Guterman; Kostya Kanyuka; Amanda J. Burridge; Andrew J. Tock; Eugenio Sanchez-M...; F. Chris H. Franklin; Ian R. Henderson; Keith J. Edwards; Cristobal Uauy; James D. Higgins
• Keywords: 2. Zero hunger; 570; 0303 health sciences; /45; Science; /631/80/641/1633; Q; article; /631/208/1405; /45/77; Article; /14/32; Meiosis; Plant Breeding; 03 medical and health sciences; /631/449/2491; /14/63; Crossing Over, Genetic; Triticum

Abstract FANCM suppresses crossovers in plants by unwinding recombination intermediates. In wheat, are skewed toward the chromosome ends, thus limiting generation of novel allelic combinations. Here, we observe that maintains obligate crossover tetraploid and hexaploid ensuring every pair exhibits at least one crossover, localizing class I protein HEI10 pachytene. also II increased 2.6-fold fancm msh5 quadruple mutants. These data consistent with a role for second-end capture designated sites, whilst is required to promote formation non-crossovers. genetic mapping reveals increase 31% compared wild type, indicating could be an effective tool accelerate breeding. Crossover rate differences correlate type distributions, suggesting chromatin may influence landscape similar ways both .