Comparative genomics of Acinetobacter baumannii and therapeutic bacteriophages from a patient undergoing phage therapy

• DOI: 10.1038/s41467-022-31455-5
• Publication Date: 2022-06-30T14:04:57Z
• Authors: Mei Liu; Adriana Hernandez...; James Clark; Tram Le; Biswajit Biswas; Kimberly A. Bisho...; Matthew Henry; Javier Quinones; Logan J. Voegtly; Regina Z. Cer; Theron Hamilton; Robert T. Schooley; Scott Salka; Ry Young; Jason J. Gill
• Keywords: Lytic cycle; Acinetobacter baumannii; Phage therapy; Strain (injury)

Abstract In 2016, a 68-year-old patient with disseminated multidrug-resistant Acinetobacter baumannii infection was successfully treated using lytic bacteriophages. Here we report the genomes of nine phages used for treatment and three strains A. isolated prior to during treatment. The in initial are related, T4-like myophages. Analysis 19 isolates collected before phage shows that resistance appeared two days following start We generate complete genomic sequences (TP1, TP2 TP3) treatment, supporting clonal relationship. Furthermore, use strain TP1 select increased five vitro, identify mutations also found phage-insensitive TP3 (which evolved vivo treatment). These results support vitro investigations can produce relevant environment.