PIM1 promotes hepatic conversion by suppressing reprogramming-induced ferroptosis and cell cycle arrest

• DOI: 10.1038/s41467-022-32976-9
• Publication Date: 2022-09-06T06:02:43Z
• Authors: Yangyang Yuan; Chenwei Wang; Xuran Zhuang; Shaofeng Lin; Miaomiao Luo; Wankun Deng; Jiaqi Zhou; Lihui Liu; Lina Mao; Wenbo Peng; Jian Chen; Qiangsong Wang; Yilai Shu; Yu Xue; Pengyu Huang
• Keywords: Reprogramming

Protein kinase-mediated phosphorylation plays a critical role in many biological processes. However, the identification of key regulatory kinases is still great challenge. Here, we develop trans-omics-based method, central kinase inference, to predict potentially by integrating quantitative transcriptomic and phosphoproteomic data. Using known associated with anti-cancer drug resistance, accuracy our method denoted area under curve 5.2% 29.5% higher than Kinase-Substrate Enrichment Analysis. We further use this analyze trans-omic data hepatocyte maturation hepatic reprogramming human dermal fibroblasts, uncovering 5 as regulators two Further experiments reveal that serine/threonine kinase, PIM1, promotes conversion protects fibroblasts from reprogramming-induced ferroptosis cell cycle arrest. This study not only reveals new kinases, but also provides helpful might be extended involved other