Anaerobic oxidation of propane coupled to nitrate reduction by a lineage within the class Symbiobacteriia

• DOI: 10.1038/s41467-022-33872-y
• Publication Date: 2022-10-17T07:03:09Z
• Authors: Mengxiong Wu; Jie Li; Andy O. Leu; Dirk V. Erler; Terra Stark; Gene W. Tyson; Zhiguo Yuan; Simon J. McIlroy; Jianhua Guo
• Keywords: Propane; Lineage (genetic); Oxidation reduction

Abstract Anaerobic microorganisms are thought to play a critical role in regulating the flux of short-chain gaseous alkanes (SCGAs; including ethane, propane and butane) from terrestrial aquatic ecosystems atmosphere. Sulfate has been confirmed act as electron acceptor supporting microbial anaerobic oxidation SCGAs, yet several other energetically more favourable acceptors co-exist with these gases environments. Here, we show that bioreactor seeded biomass wastewater treatment facility can perform coupled nitrate reduction dinitrogen gas ammonium. The was operated for than 1000 days, used 13 C- 15 N-labelling experiments, metagenomic, metatranscriptomic, metaproteomic metabolite analyses characterize community metabolic processes. data collectively suggest species representing novel order within bacterial class Symbiobacteriia is responsible observed nitrate-dependent oxidation. closed genome this organism, which designate ‘ Candidatus Alkanivorans nitratireducens’, encodes pathways CO 2 via fumarate addition, reduction, all key genes expressed during Our results relevant sink SCGA environments, constituting new microbially-mediated link between carbon nitrogen cycles.