Protective effects of macrophage-specific integrin α5 in myocardial infarction are associated with accentuated angiogenesis

• DOI: 10.1038/s41467-023-43369-x
• Publication Date: 2023-11-20T20:02:22Z
• Authors: Ruoshui Li; Bijun Chen; Akihiko Kubota; Anis Hanna; Claudio Humeres; Silvia C. Hernandez; Yang Liu; Richard Ma; Izabela Tuleta; Shuaibo Huang; Harikrishnan Venu...; Fenglan Zhu; Kai Su; Jun Li; Jinghang Zhang; Deyou Zheng; Nikolaos G. Frang...
• Keywords: Integrin-linked kinase

Abstract Macrophages sense changes in the extracellular matrix environment through integrins and play a central role regulation of reparative response after myocardial infarction. Here we show that macrophage integrin α5 protects infarcted heart from adverse remodeling protective actions are associated with acquisition an angiogenic phenotype. We demonstrate myeloid cell- macrophage-specific knockout mice have accentuated post-infarction remodeling, accompanied by reduced angiogenesis infarct border zone. Single cell RNA-sequencing identifies population high Itga5 expression. The effects macrophages involve upregulation Vascular Endothelial Growth Factor A. transcriptome vivo vitro followed bioinformatic analysis several intracellular kinases as potential downstream targets α5. Neutralization assays α5-stimulated activation Focal Adhesion Kinase Phosphoinositide 3 cascades.