Exploring the gut DNA virome in fecal immunochemical test stool samples reveals associations with lifestyle in a large population-based study

• DOI: 10.1038/s41467-024-46033-0
• Publication Date: 2024-02-29T11:02:17Z
• Authors: Paula Istvan; Einar Birkeland; Ekaterina Avershina; Ane S. Kværner; Vahid Bemanian; Barbara Pardini; Sonia Tarallo; Willem M. de Vos; Torbjørn Rognes; Paula Berstad; Trine B. Rounge
• Keywords: Human virome

Stool samples for fecal immunochemical tests (FIT) are collected in large numbers worldwide as part of colorectal cancer screening programs. Employing FIT from 1034 CRCbiome participants, recruited a Norwegian study, we identify, annotate and characterize more than 18000 DNA viruses, using shotgun metagenome sequencing. Only six percent them assigned to known taxonomic family, with Microviridae being the most prevalent viral family. Linking individual profiles comprehensive lifestyle demographic data shows 17/25 variables be associated gut virome. Physical activity, smoking, dietary fiber consumption exhibit strong consistent associations both diversity relative abundance well enrichment auxiliary metabolic genes. We demonstrate suitability virome analysis, opening an opportunity large-scale studies this enigmatic microbiome. The diverse populations their connections uncovered herein paves way further exploration role health disease.