Atypical heat shock transcription factor HSF5 is critical for male meiotic prophase under non-stress conditions

• DOI: 10.1038/s41467-024-47601-0
• Publication Date: 2024-04-29T10:01:59Z
• Authors: Saori Yoshimura; Ryuki Shimada; Koji Kikuchi; Soichiro Kawagoe; Hironori Abe; Sakie Iisaka; Sayoko Fujimura; Kei-ichiro Yasunaga; Shingo Usuki; Naoki Tani; Takashi Ohba; Eiji Kondoh; Tomohide Saio; Kimi Araki; Kei-ichiro Ishiguro
• Keywords: Heat shock factor

Abstract Meiotic prophase progression is differently regulated in males and females. In males, pachytene transition during meiotic accompanied by robust alteration gene expression. However, how expression to ensure completion remains elusive. Herein, we identify HSF5 as a male germ cell-specific heat shock transcription factor (HSF) for progression. Genetic analyzes single-cell RNA-sequencing demonstrate that essential beyond the stage under non-stress conditions rather than stress. Chromatin binding analysis vivo DNA-binding assays vitro suggest binds promoters subset of genes associated with chromatin organization. recognizes DNA motif different from typical elements recognized other canonical HSFs. This study suggests an atypical HSF required program males.