Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection

• DOI: 10.1038/s41467-024-47963-5
• Publication Date: 2024-05-01T12:52:39Z
• Authors: Sungmin Moon; Seunghan Han; In-Hwan Jang; Jaechan Ryu; Min-Seok Rha; Hyung-Ju Cho; Sang Sun Yoon; Ki Taek Nam; Chang-Hoon Kim; Man-Seong Park; Je Kyung Seong; Won-Jae Lee; Joo-Heon Yoon; Youn Wook Chung; Ji-Hwan Ryu
• Keywords: CD47

Respiratory viral infection increases host susceptibility to secondary bacterial infections, yet the precise dynamics within airway epithelia remain elusive. Here, we elucidate pivotal role of CD47 in epithelium during super-infection. We demonstrated that upon influenza virus infection, expression was upregulated and localized on apical surface ciliated cells primary human nasal or bronchial epithelial cells. This induced exposure provided attachment sites for Staphylococcus aureus, thereby compromising barrier integrity. Through adhesion assays vitro pull-down assays, identified fibronectin-binding proteins (FnBP) S. aureus as a key component binds CD47. Furthermore, found cell-specific deficiency neutralizing antibody-mediated inactivation enhanced vivo survival rates. These findings suggest interfering with interaction between pathogenic FnBP holds promise alleviating adverse effects