High throughput platform technology for rapid target identification in personalized phage therapy

• DOI: 10.1038/s41467-024-49710-2
• Publication Date: 2024-07-11T10:02:48Z
• Authors: Fereshteh Bayat; Arwa Hilal; Mathura Thirugnan...; Denise Tremblay; Carlos D. M. Filipe; Sylvain Moineau; Tohid F. Didar; Zeinab Hosseinidoust
• Keywords: Phage therapy; Salmonella enterica

Abstract As bacteriophages continue to gain regulatory approval for personalized human therapy against antibiotic-resistant infections, there is a need transformative technologies rapid target identification through multiple, large, decentralized therapeutic phages biobanks. Here, we design high throughput phage screening platform comprised of portable library individual shelf-stable, ready-to-use phages, in all-inclusive solid tablets. Each tablet encapsulates one along with luciferin and luciferase enzyme stabilized sugar matrix pullulan trehalose capable directly detecting phage-mediated adenosine triphosphate (ATP) release ATP bioluminescence reaction upon bacterial cell burst. The composition also enhances desiccation tolerance all components, which should allow easier cheaper international transportation as result, increased accessibility phages. We demonstrate by identifying select multidrug-resistant clinical isolates Pseudomonas aeruginosa , Salmonella enterica Escherichia coli Staphylococcus aureus targets identified within 30-120 min.