Atypical and non-classical CD45RBlo memory B cells are the majority of circulating SARS-CoV-2 specific B cells following mRNA vaccination or COVID-19

• DOI: 10.1038/s41467-024-50997-4
• Publication Date: 2024-08-09T11:01:44Z
• Authors: David G. Priest; Takeshi Ebihara; Janyerkye Tulyeu; Jonas N. Søndergaard; Shuhei Sakakibara; Fuminori Sugihara; Shunichiro Nakao; Yuki Togami; Jumpei Yoshimura; Hiroshi Ito; Shinya Onishi; Arisa Muratsu; Yumi Mitsuyama; Hiroshi Ogura; Jun Oda; Daisuke Okusaki; Hisatake Matsumoto; James B. Wing
• Keywords: 2019-20 coronavirus outbreak; Sars virus

Resting memory B cells can be divided into classical or atypical groups, but the heterogenous marker expression on activated makes similar classification difficult. Here, by longitudinal analysis of mass cytometry and CITE-seq data from cohorts with COVID-19, bacterial sepsis, BNT162b2 mRNA vaccine, we observe that resting cell consist CD45RB+ CD45RBlo memory, which latter contains two distinct groups CD11c+ CD23+ non-classical cells. CD45RB levels remain stable in these after activation, thereby enabling tracking plasmablasts derived either Moreover, both COVID-19 patients vaccination, formed majority SARS-CoV2 specific correlated serum antibodies, while are sepsis. Our results thus identify stably expressed exploited to trace their progeny, suggest contribute SARS-CoV-2 infection vaccination.