Systematic biotechnological production of isoprenoid analogs with bespoke carbon skeletons

• DOI: 10.1038/s41467-025-57494-2
• Publication Date: 2025-03-01T13:55:20Z
• Authors: Lina Wang; Mads Rosenfeldt; Aikaterini Koutsa...; Maria Harizani; Yong Zhao; Nattawat Leelahakorn; Axelle Frachon; Morten H. Raadam; Karel Miettinen; Irini Pateraki; Efstathia Ioannou; Sotirios C. Kampr...
• Keywords: Bespoke

Natural products are widely used as pharmaceuticals, flavors, fragrances, and cosmetic ingredients. Synthesizing evaluating analogs of natural can considerably expand their applications. However, the chemical synthesis is severely hampered by highly complex structures. This particularly evident in isoprenoids, largest class products. Here, we develop a yeast cell-based biocatalytic method that enables systematic biotechnological production different classes isoprenoids (including monoterpenoids, sesquiterpenoids, triterpenoids, cannabinoids) with additional carbons skeletons. We demonstrate applicability this approach through two proof-of-concept studies: biosynthesis valued aroma ingredient ethyllinalool, cannabinoid improved receptor agonism. simple, readily adaptable to any cell factory, tailored expansion isoprenoid space identify molecules properties valuable compounds. Current efforts on bioproduction mainly based adding functional groups existing core scaffolds. authors enable