Rapid and Integrated Bacterial Evolution Analysis unveils gene mutations and clinical risk of Klebsiella pneumoniae

• DOI: 10.1038/s41467-025-58049-1
• Publication Date: 2025-03-26T01:25:50Z
• Authors: Kojiro Uemura; Toyotaka Sato; Soh Yamamoto; Noriko Ogasawara; Jirachaya Toyting; Kotaro Aoki; Akira Takasawa; Masayuki Koyama; Atsushi Saito; Takayuki Wada; Kaho Okada; Yurie Yoshida; Koji Kuronuma; Chie Nakajima; Yasuhiko Suzuki; Motohiro Horiuchi; Kenichi Takano; Satoshi Takahashi; Hirofumi Chiba; Shin-ichi Yokota
• Keywords: Klebsiella

Bacteria continually evolve. Previous studies have evaluated bacterial evolution in retrospect, but this approach is based on only speculation. Cohort are reliable require a long duration. Additionally, identifying which genetic mutations that emerged during possess functions of interest to researchers an exceptionally challenging task. Here, we establish Rapid and Integrated Bacterial Evolution Analysis (RIBEA) serial passaging experiments using hypermutable strains, whole-genome transposon-directed sequencing, vivo evaluations monitor cohort for one month. RIBEA reveals factors contributing serum antimicrobial resistance by gene occurred the major respiratory pathogen Klebsiella pneumoniae. also enables evaluation risk progression development invasive ability from lung blood resistance. Our results demonstrate observation prediction identification clinically relevant high-risk clarifying associated pathogenicity at mutation level. Authors develop present (Rapid Analysis), combines lab-based experiments, genome sequencing testing, long-term or resistance, context