The reference genome of Miscanthus floridulus illuminates the evolution of Saccharinae

• DOI: 10.1038/s41477-021-00908-y
• Publication Date: 2021-05-06T16:03:37Z
• Authors: Guobin Zhang; Chunxia Ge; Pingping Xu; Shukai Wang; Senan Cheng; Yanbin Han; Yancui Wang; Yongbin Zhuang; Xinwei Hou; Ting Yu; Xitong Xu; Shuhan Deng; Quanquan Li; Yinqing Yang; Xiaoru Yin; Weidong Wang; Wenxue Liu; Chunxiao Zheng; Xuezhen Sun; Zhenlin Wang; Ray Ming; Shuting Dong; Jianxin Ma; Xiansheng Zhang; Cuixia Chen
• Keywords: 2. Zero hunger; Sequence Analysis, DNA; Poaceae; Synteny; 7. Clean energy; Article; Chromosomes, Plant; Saccharum; Evolution, Molecular; Genetics, Population; Glucosyltransferases; Gene Duplication; Sequence Alignment; Genome, Plant; Phylogeny; Sorghum

Abstract Miscanthus , a member of the Saccharinae subtribe that includes sorghum and sugarcane, has been widely studied as feedstock for cellulosic biofuel production. Here, we report sequencing assembly floridulus genome by integration PacBio Hi-C mapping, resulting in chromosome-scale, high-quality reference genus . Comparisons among genomes suggest Sorghum split first from common ancestor Saccharum which subsequently diverged each other, with two successive whole-genome duplication events occurring independently one genus. Fusion chromosomes occurred during rediploidization M. no significant subgenome dominance was observed. A survey cellulose synthases (CesA) revealed quite high expression most CesA genes growing stems, is agreement content this species. Resequencing comparisons 75 accessions lutarioriparius genetically close to sacchariflorus more distantly related other species diverse. This study provides valuable genomic resource molecular breeding improvement crops.