Pulcherrimin protects Bacillus subtilis against oxidative stress during biofilm development

• DOI: 10.1038/s41522-023-00418-z
• Publication Date: 2023-07-19T19:06:06Z
• Authors: Leticia Lima Ange...; Renato Augusto Co...; Gabriel Fox; Srinand Paruthiyil; Kevin Gozzi; Moshe Shemesh; Yunrong Chai
• Keywords: Microbial ecology; Oxidative Stress; Iron; Biofilms; QR100-130; Reactive Oxygen Species; Article; Bacillus subtilis

Pulcherrimin is an iron-binding reddish pigment produced by various bacterial and yeast species. In the soil bacterium Bacillus subtilis, this synthesized intracellularly as colorless pulcherriminic acid using two molecules of tRNA-charged leucine substrate; are then secreted bind to ferric iron extracellularly form red-colored pulcherrimin. The biological importance pulcherrimin not well understood. A previous study showed that secretion caused depletion in surroundings growth arrest on cells located at edge a B. subtilis colony biofilm. study, we identified primarily under biofilm conditions provides protection against oxidative stress. We presented molecular evidence how lowers level reactive oxygen species (ROS) alleviates stress DNA damage ROS accumulation mature also performed global transcriptome profiling identify differentially expressed genes pulcherrimin-deficient mutant compared with wild type, further characterized regulation related homeostasis, response (DDR), response. Based our findings, propose important antioxidant modulates development.