Proteomic analysis of human mesenchymal stromal cell secretomes: a systematic comparison of the angiogenic potential

• DOI: 10.1038/s41536-019-0070-y
• Publication Date: 2019-04-16T10:03:21Z
• Authors: Debora Kehl; Melanie Generali; Anna Mallone; Manfred Heller; Anne-Christine Uldry; Phil Cheng; Benjamin Gantenbein; Simon P. Hoerstrup; Benedikt Weber
• Keywords: 1307 Cell Biology; 1309 Developmental Biology; 2204 Biomedical Engineering; 2701 Medicine (miscellaneous); 610 Medicine & health; 11359 Institute for Regenerative Medicine (IREM); Article

Human mesenchymal stromal cell (hMSC) secretomes have shown to influence the microenvironment upon injury, promoting cytoprotection, angiogenesis, and tissue repair. The angiogenic potential is of particular interest for treatment ischemic diseases. Interestingly, hMSC isolated from different sources dissimilarities with respect their profile. This study compares angiogenesis adipose (hADSCs), bone marrow (hBMSCs), umbilical cord Wharton's jelly (hWJSCs). were obtained under xenofree conditions analyzed by liquid chromatography tandem mass spectrometry (LC/MS-MS). Biological processes related found be enriched in proteomic profile secretomes. hWJSC revealed a more complete network higher concentrations proteins, followed hBMSC hADSC lacked central proteins expressed most detected significantly lower level. In vivo all induced vascularization subcutaneously implanted Matrigel plugs mice. Differences secretome composition functionally monocyte endothelial (EC) vitro co-culture experiments using vi-SNE based multidimensional flow cytometry data analysis. Functional responses between comparable, migration CD14++ CD16- monocytes enhanced macrophage differentiation compared Both also profound pro-angiogenic phenotype ECs. These results suggest hWJSCs as potent source inflammation-mediated induction, while potency was lowest. systematic analysis may implication on selection hMSCs future clinical studies.