Improved reference genome of Aedes aegypti informs arbovirus vector control

• DOI: 10.1038/s41586-018-0692-z
• Publication Date: 2018-11-14T11:10:55Z
• Authors: Benjamin J. Matthews; Olga Dudchenko; Sarah B. Kingan; Sergey Koren; Igor Antoshechkin; Jacob E. Crawford; William J. Glassford; Margaret Herre; Seth N. Redmond; Noah H. Rose; Gareth D. Weedall; Yang Wu; Sanjit S. Batra; Carlos A. Brito-S...; Steven D. Buckingham; Corey L. Campbell; Saki Chan; Eric Cox; Benjamin R. Evans; Thanyalak Fansiri; Igor Filipović; Albin Fontaine; Andrea Gloria-Soria; Richard Hall; Vinita S. Joardar; Andrew K. Jones; Raissa G. G. Kay; Vamsi K. Kodali; Joyce Lee; Gareth J. Lycett; Sara N. Mitchell; Jill Muehling; Michael R. Murphy; Arina D. Omer; Frederick A. Part...; Paul Peluso; Aviva Presser Aiden; Vidya Ramasamy; Gordana Rašić; Sourav Roy; Karla Saavedra-Ro...; Shruti Sharan; Atashi Sharma; Melissa Laird Smith; Joe Turner; Allison M. Weakley; Zhilei Zhao; Omar S. Akbari; William C. Black; Han Cao; Alistair C. Darby; Catherine A. Hill; J. Spencer Johnston; Terence D. Murphy; Alexander S. Raikhel; David B. Sattelle; Igor V. Sharakhov; Bradley J. White; Li Zhao; Erez Lieberman Aiden; Richard S. Mann; Louis Lambrechts; Jeffrey R. Powell; Maria V. Sharakhova; Zhijian Tu; Hugh M. Robertson; Carolyn S. McBride; Alex R. Hastie; Jonas Korlach; Daniel E. Neafsey; Adam M. Phillippy; Leslie B. Vosshall
• Keywords: Male; 0301 basic medicine; Population genetics; [SDV]Life Sciences [q-bio]; Genome, Insect; Gene; Insecticide Resistance; Agricultural and Biological Sciences; Aedes aegypti; Sociology; Aedes; Pyrethrins; 2.2 Factors relating to the physical environment; Vector (molecular biology); Glutathione Transferase; Arbovirus; Multidisciplinary; Genome; Ecology; Life Sciences; Genomics; Biological Sciences; Reference Standards; 16. Peace & justice; Virus; FOS: Sociology; 3. Good health; Infectious Diseases; Multigene Family; Plant-insect interactions; Larva; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology; Medicine; Female; Insect Symbiosis and Microbial Interactions; Yellow fever; Infection; 570; DNA Copy Number Variations; General Science & Technology; Population; Bioinformatics and Computational Biology; 610; Mosquito Vectors; Arbovirus Infections; Insect Control; Article; QH301; 03 medical and health sciences; Rare Diseases; Biodefense; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]; Biochemistry, Genetics and Molecular Biology; Virology; Health Sciences; Genetics; Animals; Global Impact of Arboviral Diseases; Molecular Biology; Biology; Demography; QR355; 3.2 Interventions to alter physical and biological environmental risks; [SDV.GEN]Life Sciences [q-bio]/Genetics; Recombinant DNA; Public Health, Environmental and Occupational Health; Genetic Variation; Molecular Sequence Annotation; Sex Determination Processes; Dengue Virus; Dengue fever; Vector-Borne Diseases; Molecular Mechanisms of Insect Resistance to Xenobiotics; Emerging Infectious Diseases; Good Health and Well Being; Genetics, Population; Insect Science; FOS: Biological sciences; Locus (genetics); Chikungunya; Insect; Arboviruses; [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis

Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of developing tools to fight them has been slowed by lack a high-quality genome assembly. Here we combine diverse technologies produce markedly improved, fully re-annotated AaegL5 assembly, demonstrate how it accelerates mosquito science. We anchored physical cytogenetic maps, doubled number known chemosensory ionotropic receptors that guide human hosts egg-laying sites, provided further insight into size composition sex-determining M locus, revealed copy-number variation among glutathione S-transferase genes are important for insecticide resistance. Using high-resolution quantitative trait locus population genomic analyses, mapped new candidates dengue vector competence will catalyse biological insights intervention strategies this deadly disease vector.