Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase

• DOI: 10.1038/s41587-022-01595-6
• Publication Date: 2023-01-09T17:02:57Z
• Authors: Huawei Tong; Xuchen Wang; Yuanhua Liu; Nana Liu; Yun Li; Jiamin Luo; Qian Ma; Danni Wu; Jiyong Li; Chunlong Xu; Hui Yang
• Keywords: Transversion; Base (topology)

Here we developed an adenine transversion base editor, AYBE, for A-to-C and A-to-T transversion editing in mammalian cells by fusing an adenine base editor (ABE) with hypoxanthine excision protein N-methylpurine DNA glycosylase (MPG). We also engineered AYBE variants enabling targeted editing at genomic loci with higher transversion editing activity (up to 72% for A-to-C or A-to-T editing).