X-ray ptychographic and fluorescence microscopy of frozen-hydrated cells using continuous scanning

• DOI: 10.1038/s41598-017-00569-y
• Publication Date: 2017-03-21T23:16:25Z
• Authors: Junjing Deng; David J. Vine; Si Chen; Qiaoling Jin; Youssef S. G. Nashed; Tom Peterka; Stefan Vogt; Chris Jacobsen
• Keywords: Fluorescence-lifetime imaging microscopy; Biological specimen

X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron for submicrometer thick specimens, and molecule-specific imaging capabilites fluorescence light microscopy. We describe here first use fast, continuous x-ray scanning frozen simultaneous sub-20 nm ptychographic transmission with high contrast, sub-100 deconvolved diffusible bound ions at concentrations, without need add specific labels. By working that have been rapidly chemical fixatives, them under cryogenic conditions, we are able obtain images well preserved structural composition, sufficient stability against radiation damage allow multiple obtained no observable change.