Comparative Transcriptome Analysis Identified Candidate Genes Related to Bailinggu Mushroom Formation and Genetic Markers for Genetic Analyses and Breeding

• DOI: 10.1038/s41598-017-08049-z
• Publication Date: 2017-08-17T16:55:50Z
• Authors: Yongping Fu; Yueting Dai; Chentao Yang; Peng Wei; Bing Song; Yang Yang; Lei Sun; Zhi-Wu Zhang; Yu Li
• Keywords: Germ plasm; Molecular breeding; Candidate gene

Bailinggu (Pleurotus tuoliensis) is a major, commercially cultivated mushroom and widely used for nutritional, medicinal, industrial applications. Yet, the mushroom's genetic architecture molecular mechanisms underlying its formation are largely unknown. Here we performed comparative transcriptomic analysis during Bailinggu's mycelia, primordia, fruiting body stages to identify genes regulating development develop EST-SSR markers assessing value of breeding materials. The stage-specific differentially expressed unigenes (DEGs) involved in morphogenesis, primary carbohydrate metabolism, cold stimulation blue-light response were identified using GO KEGG databases. These might help adapt environmental factors that influence fructification. most pronounced change gene expression occurred vegetative-to-reproductive transition, suggesting active key development. We then developed 26 polymorphic informative assess diversity 82 strains EST-SSRs exhibited high transferability closely related species P. eryngii var. ferulae eryngii. Genetic population structure indicated China's has low introgression with these two varieties likely evolved independently. findings provide new genes, SSR markers, germplasm enhance Bailinggu.