Functional characterization of the Ucp1-associated oxidative phenotype of human epicardial adipose tissue
Male
Proteomics
0303 health sciences
Biopsy
Primary Cell Culture
Isoproterenol
Middle Aged
Article
Body Mass Index
Oxidative Stress
03 medical and health sciences
Adipocytes, Brown
Oxygen Consumption
Phenotype
Adipose Tissue
Adipose Tissue, Brown
Gene Expression Regulation
Humans
Female
Insulin Resistance
Oxidation-Reduction
Pericardium
Aged
DOI:
10.1038/s41598-017-15501-7
Publication Date:
2017-11-08T14:42:36Z
AUTHORS (8)
ABSTRACT
AbstractBrown fat presence and metabolic activity has been associated with lower body mass index, higher insulin sensitivity and better cardiometabolic profile in humans. We, and others, have previously reported the presence of Ucp1, a marker of brown adipocytes, in human epicardial adipose tissue (eAT). Characterization of the metabolic activity and associated physiological relevance of Ucp1 within eAT, however, is still awaited. Here, we validate the presence of Ucp1 within human eAT and its ‘beige’ nature. Using in-vitro analytical approaches, we further characterize its thermogenic potential and demonstrate that human eAT is capable of undergoing enhanced uncoupling respiration upon stimulation. Direct biopsy gene expression analysis reveals a negative association between thermogenic markers and oxidative stress-related genes in this depot. Consistently, isoproterenol (Iso) stimulation of eAT leads to a downregulation of secreted proteins included in the GO terms ‘cell redox homeostasis’ and ‘protein folding’. In addition, cardiac endothelial cells exhibit a downregulation in the expression of adhesion markers upon treatment with Iso-stimulated eAT derived conditioned media. Overall, these observations suggest that Ucp1- associated metabolic activity plays a significant role in local tissue homeostasis within eAT and can plausibly alter its communication with neighboring cells of the cardiovascular system.
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