Functional characterization of the Ucp1-associated oxidative phenotype of human epicardial adipose tissue

• DOI: 10.1038/s41598-017-15501-7
• Publication Date: 2017-11-08T14:42:36Z
• Authors: Kanta Chechi; Pierre Voisine; Patrick Mathieu; Mathieu Laplante; Sébastian Bonnet; Frédéric Picard; Philippe Joubert; Denis Richard
• Keywords: Thermogenin; Homeostasis

Brown fat presence and metabolic activity has been associated with lower body mass index, higher insulin sensitivity better cardiometabolic profile in humans. We, others, have previously reported the of Ucp1, a marker brown adipocytes, human epicardial adipose tissue (eAT). Characterization physiological relevance Ucp1 within eAT, however, is still awaited. Here, we validate eAT its 'beige' nature. Using in-vitro analytical approaches, further characterize thermogenic potential demonstrate that capable undergoing enhanced uncoupling respiration upon stimulation. Direct biopsy gene expression analysis reveals negative association between markers oxidative stress-related genes this depot. Consistently, isoproterenol (Iso) stimulation leads to downregulation secreted proteins included GO terms 'cell redox homeostasis' 'protein folding'. In addition, cardiac endothelial cells exhibit adhesion treatment Iso-stimulated derived conditioned media. Overall, these observations suggest Ucp1- plays significant role local homeostasis can plausibly alter communication neighboring cardiovascular system.