Differential long non-coding RNA expression profiles in human oocytes and cumulus cells

• DOI: 10.1038/s41598-018-20727-0
• Publication Date: 2018-01-29T11:40:01Z
• Authors: Julien Bouckenheimer; Patricia Fauque; Charles-Henri Lec...; Céline Bruno; Thérèse Commes; Jean-Marc Lemaître; John De Vos; Said Assou
• Keywords: 0301 basic medicine; Cumulus Cells; Reverse Transcriptase Polymerase Chain Reaction; [SDV]Life Sciences [q-bio]; Gene Expression Profiling; Computational Biology; Real-Time Polymerase Chain Reaction; Article; 3. Good health; [SDV] Life Sciences [q-bio]; 03 medical and health sciences; Oocytes; Humans; RNA, Long Noncoding; Metaphase

AbstractProgress in assisted reproductive technologies strongly relies on understanding the regulation of the dialogue between oocyte and cumulus cells (CCs). Little is known about the role of long non-coding RNAs (lncRNAs) in the human cumulus-oocyte complex (COC). To this aim, publicly available RNA-sequencing data were analyzed to identify lncRNAs that were abundant in metaphase II (MII) oocytes (BCAR4, C3orf56, TUNAR, OOEP-AS1, CASC18, and LINC01118) and CCs (NEAT1, MALAT1, ANXA2P2, MEG3, IL6STP1, and VIM-AS1). These data were validated by RT-qPCR analysis using independent oocytes and CC samples. The functions of the identified lncRNAs were then predicted by constructing lncRNA-mRNA co-expression networks. This analysis suggested that MII oocyte lncRNAs could be involved in chromatin remodeling, cell pluripotency and in driving early embryonic development. CC lncRNAs were co-expressed with genes involved in apoptosis and extracellular matrix-related functions. A bioinformatic analysis of RNA-sequencing data to identify CC lncRNAs that are affected by maternal age showed that lncRNAs with age-related altered expression in CCs are essential for oocyte growth. This comprehensive analysis of lncRNAs expressed in human MII oocytes and CCs could provide biomarkers of oocyte quality for the development of non-invasive tests to identify embryos with high developmental potential.