Luminescent and paramagnetic properties of nanoparticles shed light on their interactions with proteins

• DOI: 10.1038/s41598-018-21571-y
• Publication Date: 2018-02-15T16:47:09Z
• Authors: Giuditta Dal Cortivo; Gabriel E. Wagner; Paolo Cortelletti; Krishna Mohan Pad...; Klaus Zangger; Adolfo Speghini; Daniele Dell’Orco; N. Helge Meyer
• Keywords: Models, Molecular; 0301 basic medicine; Luminescence; Protein Conformation; Protein Stability; Circular Dichroism; Surface Plasmon Resonance; Article; Calcium Fluoride; 03 medical and health sciences; Calmodulin; nanoscale biophysics; nanoscale devices; Medicine and health; Humans; Nanoparticles; EF Hand Motifs; Nuclear Magnetic Resonance, Biomolecular; Protein Binding

Abstract Nanoparticles have been recognized as promising tools for targeted drug-delivery and protein therapeutics. However, the mechanisms of protein-nanoparticle interaction dynamics underlying binding process are poorly understood. Here, we present a general methodology characterization on molecular level. To this end combined biophysical techniques including nuclear magnetic resonance (NMR), circular dichroism (CD), energy transfer (RET) surface plasmon (SPR). Particularly, analyzed CaF 2 nanoparticles with prototypical calcium sensor calmodulin (CaM). We observed transient formation an intermediate encounter complex involving structural region linking two domains. Specific CaM NPs is driven by N-terminal EF-hands, which seem to recognize Ca 2+ nanoparticle. conclude that NP-CaM fully compatible potential applications in nanomedicine. Overall, methods presented work can be extended other systems may useful quantitatively characterize dynamic features protein-NP interactions important implications nanomedicine nano-biotechnology.