Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis

• DOI: 10.1038/s41598-018-25676-2
• Publication Date: 2018-05-04T14:22:47Z
• Authors: Alessia Farinazzo; Stefano Angiari; Ermanna Turano; Edoardo Bistaffa; Silvia Dusi; Serena Ruggieri; Roberta Bonafede; Raffaella Mariotti; Gabriela Constantin; Bruno Bonetti
• Keywords: Encephalomyelitis

Cell based-therapies represent promising strategies for the treatment of neurological diseases. We have previously shown that adipose stem cells (ASC) ameliorate chronic experimental autoimmune encephalomyelitis (EAE). Recent evidence indicates most ASC paracrine effects are mediated by extracellular vesicles, i.e. micro- and nanovesicles (MVs NVs). show preventive intravenous administration NVs isolated from (ASC-NVs) before disease onset significantly reduces severity EAE decreases spinal cord inflammation demyelination, whereas therapeutic with ASC-NVs does not established EAE. This marginally inhibits antigen-specific T cell activation, while reducing microglial activation demyelination in cord. Importantly, inhibited integrin-dependent adhesion encephalitogenic vitro, no effect on molecule expression. In addition, intravital microscopy showed treated display a reduced rolling firm inflamed vessels compared to untreated cells. Our results pathogenesis mainly inhibiting extravasation CNS, suggesting may novel approach neuro-inflammatory diseases, enabling safe effector factors.