Transcriptome analysis of the anhydrobiotic cell line Pv11 infers the mechanism of desiccation tolerance and recovery

• DOI: 10.1038/s41598-018-36124-6
• Publication Date: 2018-12-12T11:30:36Z
• Authors: Takahiro G. Yamada; Yoshitaka Suetsugu; Ruslan Deviatiiarov; Oleg Gusev; Richard Cornette; Alexander Nesmelov; Noriko Hiroi; Takahiro Kikawada; Akira Funahashi
• Keywords: Cryptobiosis; Desiccation Tolerance

Abstract The larvae of the African midge, Polypedilum vanderplanki , can enter an ametabolic state called anhydrobiosis to overcome fatal desiccation stress. Pv11 cell line, derived from P. embryo, shows tolerance when treated with trehalose before and resumes proliferation after rehydration. However, molecular mechanisms this remain unknown. Here, we performed high-throughput CAGE-seq mRNA a differentially expressed gene analysis in trehalose-treated, desiccated, rehydrated cells, followed by ontology identified genes. We detected genes treatment involved various stress responses, detoxification harmful chemicals, regulation oxidoreduction that were upregulated. In phase, L-isoaspartyl methyltransferase heat shock proteins upregulated ribosomal downregulated. Analysis during rehydration supported notion homologous recombination, nucleotide excision repair, non-homologous recombination recovery process. This study provides initial insights into underlying extreme cells.