Structural basis for chemically-induced homodimerization of a single domain antibody

• DOI: 10.1038/s41598-019-38752-y
• Publication Date: 2019-02-12T11:05:20Z
• Authors: Jean Lesne; Hung-Ju Chang; Angelique De Visch; Matteo Paloni; Philippe Barthe; Jean-François Gui...; Pauline Mayonove; Alessandro Barducci; Gilles Labesse; Jerome Bonnet; Martin Cohen-Gonsaud
• Keywords: 0301 basic medicine; Protein Conformation; [SDV]Life Sciences [q-bio]; Article; Antibodies; 3. Good health; Structure-Activity Relationship; 03 medical and health sciences; Models, Chemical; Caffeine; Humans; Immunoglobulin Domains; Dimerization

Abstract Chemically-induced dimerization (CID) systems are essential tools to interrogate and control biological systems. AcVHH is a single domain antibody homo-dimerizing upon caffeine binding. has strong potential for clinical applications through caffeine-mediated in vivo of therapeutic gene networks. Here we provide the structural basis caffeine-induced homo-dimerization acVHH.