Molecular approaches underlying the oogenic cycle of the scleractinian coral, Acropora tenuis

• DOI: 10.1038/s41598-020-66020-x
• Publication Date: 2020-06-18T10:03:26Z
• Authors: Ee Suan Tan; Ryotaro Izumi; Yuki Takeuchi; Naoko Isomura; Akihiro Takemura
• Keywords: Vitellogenin; Yolk

Abstract This study aimed to elucidate the physiological processes of oogenesis in Acropora tenuis . Genes/proteins related were investigated: Vasa, a germ cell marker, vitellogenin (VG), major yolk protein precursor, and its receptor (LDLR). Coral branches collected monthly from coral reefs around Sesoko Island (Okinawa, Japan) for histological observation by situ hybridisation (ISH) Vasa ( AtVasa ) Low Density Lipoprotein Receptor AtLDLR genes immunohistochemistry (IHC) AtVG. immunoreactivity was detected germline cells ooplasm, whereas AtVG ooplasm putative ovarian tissues. localised located retractor muscles mesentery, mesentery tissues during vitellogenic phase, found primary oocytes. Germline expressing are present throughout year. In conclusion, has molecular roles oogenic cycle, as it determines gonadal ensures normal oocyte development, VG LDLR limited stages because they act coordination with lipoprotein transport, synthesis, incorporation into