Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens

• DOI: 10.1038/s41598-020-73683-z
• Publication Date: 2020-10-08T10:03:22Z
• Authors: Mona A. Alqahtani; Monerah R. Al Othman; Afrah E. Mohammed
• Keywords: Silver nanoparticle; Zeta potential; Pathogenic bacteria; Agar diffusion test

Abstract Recently, increase bacterial resistance to antimicrobial compounds issue constitutes a real threat human health. One of the useful materials for control is Silver nanoparticles (AgNPs). Researchers tend use biogenic agents synthesize stable and safe AgNPs. The principal aim this study was investigate ability lichen in AgNPs formation find out their suppression MDR bacteria as well cytotoxic activity. In current study, lichens ( Xanthoria parietina , Flavopunctelia flaventior ) were collected from south Kingdom Saudi Arabia. Lichens methanolic extracts used conversion Ag ions Prepared characterized by Ultraviolet–Visible (UV–Vis) Spectroscopy, Transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) Zeta potential Energy-Dispersive X-ray Spectroscopy (EDS). Secondary metabolites determined Fourier-Transform Infrared (FTIR) Gas Chromatography–Mass Spectrometry (GC–MS). antibacterial activity synergistic effect evaluated against pathogenic bacteria, including gram-positive; Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-resistant Enterococcus (VRE), gram-negative; Pseudomonas aeruginosa, Escherichia coli reference strains (ATCC) using agar disk diffusion method. Cytotoxic tested HCT 116 (Human Colorectal Cancer cell), MDA-MB-231 (Breast cancer FaDu (Pharynx cell) MTT test. TEM imaging showed well-dispersed spherical particles 1–40 nm size zeta 69–145 nm. Furthermore, FTIR GC–MS identified various chemical molecules. On other hand, highest noticed P. aeruginosa followed MRSA, VRE, E. . influence on gram-negative greater than that gram-positive with some antibiotics noted examined microbes. Moreover, higher cytotoxicity cell line relation noted. Given findings, mediated had positive antibacterial, powers. Therefore, they might be considered promising candidate combat multi-drug organisms cells.