Automated synthesis and preliminary evaluation of [18F]FDPA for cardiac inflammation imaging in rats after myocardial infarction

• DOI: 10.1038/s41598-020-75705-2
• Publication Date: 2020-10-29T11:02:57Z
• Authors: Tiantian Mou; Jing Tian; Yi Tian; Mingkai Yun; Junqi Li; Wei Dong; Xia Lu; Ziwei Zhu; Hongzhi Mi; Xiaoli Zhang; Xiang Li
• Keywords: Translocator protein; Cardiac Imaging; Imaging agent

Abstract A translocator protein 18 kDa targeted radiotracer, N,N -diethyl-2-(2-(4-[ F]fluorophenyl)-5,7-dimethylpyrazolo[1,5-a] pyrimidin-3-yl) acetamide ([ F]FDPA), was automated synthetized and evaluated for cardiac inflammation imaging. Various reaction conditions an synthesis were systematically optimized. MicroPET/CT imaging performed on normal rats with myocardial infarction (MI). Normalized SUV ratios of [ F]FDPA to 13 N]NH 3 (NSRs) in different regions calculated normalize the uptake perfusion. The amount TBAOMs volume/proportion water crucial synthesis. After optimization, total time 68 min. non-decay corrected radiochemical yields (RCYs) molar activities 19.9 ± 1.7% 169.7 46.5 GBq/μmol, respectively. In rats, showed a high stable fast clearance from other organs. MI NSRs peri-infarct infarct regions, which infiltrated massive inflammatory cells revealed by pathology, higher than that remote region (1.20 0.01 1.08 0.10 vs. 0.89 0.05, respectively). synthesized RCYs activities. It offered wide window imaging, indicating it could be potential agent.