A novel paper MAP method for rapid high resolution histological analysis

• DOI: 10.1038/s41598-021-02632-1
• Publication Date: 2021-12-02T11:03:04Z
• Authors: Mirae Lee; Jiwon Woo; Doh-Hee Kim; Yu-Mi Yang; Eunice Yoojin Lee; Jung-Hee Kim; Seok-Gu Kang; Jin-Kyung Shim; Jeong-Yoon Park
• Keywords: Male; Proteome; Science; Nude; Brain Neoplasms / metabolism; Spinal Cord Injuries / pathology*; 610; Mice, Nude; Apoptosis; Article; Imaging; Mice; 03 medical and health sciences; Imaging, Three-Dimensional; Alzheimer Disease; Tumor Cells, Cultured; Animals; Humans; Alzheimer Disease / pathology*; Glioblastoma / metabolism; Spinal Cord Injuries; Three-Dimensional / methods*; Cell Proliferation; Brain Neoplasms / pathology*; Microscopy; 0303 health sciences; Cultured; Microscopy, Confocal; Brain Neoplasms; Spinal Cord Injuries / metabolism; Q; R; Brain; Brain / metabolism*; Xenograft Model Antitumor Assays; Tumor Cells; Alzheimer Disease / metabolism; Confocal; Medicine; Proteome / analysis; Proteome / metabolism*; Glioblastoma / pathology*; Glioblastoma

AbstractThree-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities of biological-phenomena, especially with regards structural and spatial relationships and pathologlical-diagnosis. Recent advancements in tissue-clearing technology, such as Magnified Analysis of Proteome (MAP), have significantly improved our ability to study biological-structures in three-dimensional space; however, their wide applicability to a variety of tissues is limited by long incubation-times and a need for advanced imaging-systems that are not readily available in most-laboratories. Here, we present optimized MAP-based method for paper-thin samples, Paper-MAP, which allow for rapid clearing and subsequent imaging of three-dimensional sections derived from various tissues using conventional confocal-microscopy. Paper-MAP successfully clear tissues within 1-day, compared to the original-MAP, without significant differences in achieved optical-transparency. As a proof-of-concept, we investigated the vasculature and neuronal-networks of a variety of human and rodent tissues processed via Paper-MAP, in both healthy and diseased contexts, including Alzheimer’s disease and glioma.