Sensitive detection and quantification of SARS-CoV-2 in saliva
Gold standard (test)
DOI:
10.1038/s41598-021-91835-7
Publication Date:
2021-06-14T10:03:10Z
AUTHORS (27)
ABSTRACT
Abstract Saliva has significant advantages as a test medium for detection of SARS-CoV-2 infection in patients, such ease collection, minimal requirement supplies and trained personnel, safety. Comprehensive validation large cohort prospectively collected specimens with unknown status should be performed to evaluate the potential limitations saliva-based testing. We developed testing pipeline nucleic acids using real-time reverse transcription PCR (RT-PCR) droplet digital (ddPCR) readouts, measured samples from 137 outpatients tested at curbside facility 29 inpatients hospitalized COVID-19. These measurements were compared nasal swab results each patient by certified microbiology laboratory. found that our saliva positively detects 100% 93.75% patients identified positive Emergency Use Authorization (EUA) assay. Quantification viral loads ddPCR revealed an extremely wide range, 1 million-fold difference between individual patients. Our demonstrate both community screening hospital settings reliability is on par swabs detecting infected cases, higher sensitivity when combined low-abundance evade traditional methods.
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