Role of myeloid cell leptin signaling in the regulation of glucose metabolism

• DOI: 10.1038/s41598-021-97549-0
• Publication Date: 2021-09-15T10:03:59Z
• Authors: Sandra Pereira; Daemon L. Cline; Melissa Chan; Kalin Chai; Ji Soo Yoon; Shannon M. O’Dwyer; Cara E. Ellis; Maria M. Glavas; Travis D. Webber; Robert K. Baker; Suheda Erener; Scott D. Covey; Timothy J. Kieffer
• Keywords: Leptin receptor

Abstract Although innate immunity is linked to metabolic health, the effect of leptin signaling in cells from immune system on glucose homeostasis has not been thoroughly investigated. We generated two mouse models using Cre-lox methodology determine myeloid cell-specific receptor ( Lepr ) reconstitution and knockdown vivo metabolism. Male mice with Lyz2Cre + loxTB/loxTB had better glycemic control as they aged compared male whole-body transcriptional blockade − ). In contrast, females only a trend for diminished hyperglycemia after prolonged fast. During tolerance tests, males mildly improved plasma profile Cre controls while similar excursion their controls. Myeloid flox/flox did significantly alter body weight, blood glucose, insulin sensitivity, or females. Expression cytokine interleukin 10 (anti-inflammatory) tended be higher adipose tissue (p = 0.0774) 6 (pro-inflammatory) was lower < 0.05) vs. respective conclusion, lineage beneficial effects metabolism mice.