Bacterial communities associated with silage of different forage crops in Malaysian climate analysed using 16S amplicon metagenomics

• DOI: 10.1038/s41598-022-08819-4
• Publication Date: 2022-05-02T11:03:03Z
• Authors: Minhalina Badrul ...; Amalia Mohd Hashim; Nursyuhaida Mohd ...; Norafizah Abdul R...; Nur Elina Abdul M...; Chun Keat Tan; Muhamad Hazim Nazli; Nur Fatihah Mohd ...
• Keywords: Silage

Abstract Silage produced in tropical countries is prone to spoilage because of high humidity and temperature. Therefore, determining indigenous bacteria as potential inoculants important improve silage quality. This study aimed determine bacterial community functional changes associated with ensiling using amplicon metagenomics predict additives quality the Malaysian climate. Silages two forage crops (sweet corn Napier) were prepared, their fermentation properties communities analysed. After ensiling, both silages predominated by lactic acid (LAB), they exhibited good significant increment acid, reductions pH water-soluble carbohydrates, low level acetic absence propionic butyric acid. LAB consortia consisting homolactic heterolactic species proposed be for sweet Napier fermentation. Tax4fun prediction revealed metabolic pathways related activities (bacterial division, carbohydrate transport catabolism, secondary metabolite production) enriched ensiled ( p < 0.05). These results might suggest active metabolism plant carbohydrates into a usable form sustain reproduction during fermentation, yielding products such research has provided comprehensive understanding before after which can useful desirable Malaysia.